The Kit Arrived...

So the kit arrived last Friday, which was very exciting ! Some Kit information:
Name : Coliscan Easygel Specification Data
Selective for: E.Coli and other coliforms
Description: Coliscan is a clear mediu containing two chromogenic substrates. One is cleaved by the nzyme galactosidase to produce an insoluble pink compound. This causes coliform colonies to be pink/red. The second is cleaved by the enzyme glucuronidase to produce an insoluble teal compound. Using the two compounds allows easy visual distinction between general coliforms(pink/red colonies) and e.coli ( pink + teal = blue/purple colonies)
Composition: contains nutrients, inhibitors and chromogenic substrate mix. pH 7.3 + 0.2
Procedure: use standard methodology for product being tested. Incubation time and temperature 24-48hours at 35c Colors will intensidy and colony size will be larger at 36-48hours.
Interpretation: E.coli colonies will have a dark blue/ purple overall colony color. Other coliforms are pink to red in color. Proteus and Sakmonella will generallly grow as white colonies. Gram positive organisms are generally inhibited. An occasional teal colony is indicative of a non-coloiform which has the ability to produce glucuronidase but not galactosidase. A fwe strains of Salmonella and Shigella have this capability.


-Brandi